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Dose-dependent hepatotoxicity of hydrogen peroxide in HepG2 cells and its modulation by CYP450 induction

In vitro liver models combined with metabolomics approaches offer promising alternatives to animal testing in toxicology. In this study, we investigated concentration-dependent effects of hydrogen peroxide on the intra- and extracellular metabolome of HepG2 cells using 1H Nuclear Magnetic Resonance spectroscopy. 

Citation:
Jinks M, Maker GL, Davies EC, et al. Dose-dependent hepatotoxicity of hydrogen peroxide in HepG2 cells and its modulation by CYP450 induction. FEBS Open Bio. 2026;10. 

Keywords:
HepG2; cytochrome P450; hydrogen peroxide; in vitro toxicology; metabolomics; model systems

Abstract:
In vitro liver models combined with metabolomics approaches offer promising alternatives to animal testing in toxicology. In this study, we investigated concentration-dependent effects of hydrogen peroxide on the intra- and extracellular metabolome of HepG2 cells using 1H Nuclear Magnetic Resonance spectroscopy.